With the pickup / vol knob interaction, I believe the pickup inductance is a key element. Simple resistance in theory affects all frequencies equally. (I’ve heard resistors start to act funny at really high frequencies, way beyond audio).
Impedance is an odd thing since it’s usually denoted by a single resistance number. But element with inductive or capacitive qualities will have a different ‘resistance’ at different frequencies.
I highly doubt there’s any issues with using the #2 (standard) with your situation, but definitely interesting to learn the technical details. (For instance, I’m not sure if that calculated tube stage impedance has significant inductive or capacitive qualities or not, but I doubt it’s anything like pickups).