Honest question: Did the drop in volume follow when you switched tubes? If so, then bad tube. If it did, then bad tube.
Things to check would be the voltages on both sides of R11 and R12 (the main resistors to the tubes). The two voltages closest to the SushiBox label and the 1n and 470p capacitor will tell us the voltage from the banded side of the UF4007. It will help us diagnose where the issues might be.
Voltage of R11 and R12 nearest the tube socket will tell us if the 100k are okay and the correct voltages are getting to the second tube's plates correctly. Double check that they - R11 and R12 - are 100k!
Also, can you measure the voltage of the two sides of the UF4007 diode? This could have had an issue because the tubes were the wrong way and it might have pulled too much current and shorted the diode. I've had that happen before!
Another idea would be to pull the NE555 and replace it and then recheck voltages. If those still have the same problem, we might need to pull V2 and look for issues.
I have a couple more ideas to check, but really voltages help. It's something I've learned from numerous screw ups and building a few amplifiers here and there. Yes, tube amplifiers are scary and there are high voltages. Best practice -- put the negative lead in the screw hole of the enclosure and hold the red probe in your dominant hand. Put your other hand behind your back when checking voltages. This will prevent voltage from going across your arms and across your heart. After working with tube amps and getting zapped by 350VDC+, I'm cautious. But it can be solved! You'll get it solved!
Things to check would be the voltages on both sides of R11 and R12 (the main resistors to the tubes). The two voltages closest to the SushiBox label and the 1n and 470p capacitor will tell us the voltage from the banded side of the UF4007. It will help us diagnose where the issues might be.
Voltage of R11 and R12 nearest the tube socket will tell us if the 100k are okay and the correct voltages are getting to the second tube's plates correctly. Double check that they - R11 and R12 - are 100k!
Also, can you measure the voltage of the two sides of the UF4007 diode? This could have had an issue because the tubes were the wrong way and it might have pulled too much current and shorted the diode. I've had that happen before!
Another idea would be to pull the NE555 and replace it and then recheck voltages. If those still have the same problem, we might need to pull V2 and look for issues.
I have a couple more ideas to check, but really voltages help. It's something I've learned from numerous screw ups and building a few amplifiers here and there. Yes, tube amplifiers are scary and there are high voltages. Best practice -- put the negative lead in the screw hole of the enclosure and hold the red probe in your dominant hand. Put your other hand behind your back when checking voltages. This will prevent voltage from going across your arms and across your heart. After working with tube amps and getting zapped by 350VDC+, I'm cautious. But it can be solved! You'll get it solved!