I would say you have a problem somewhere in your wiring or in the signal path. Maybe a bad ground, maybe a short, maybe a wrong part, maybe a borken part,. Not really enough information in your post to help you troubleshoot your board to find the problem, which is ultimately what needs to happen.
You can read some of the other requests for help on projects to get an idea of the process to follow. I would suggest starting by taking the pcb out of your enclosure (it you already boxed it) to see if it works that way. If it doesn't leave it out of the box until you get it working.